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Figure 1: The principle of Ni-NTA affinity purification FPLC exploits the Ni-NTA affinity chromatography technique to purify His6-tagged proteins Fast Pressure Liquid Chromatography (FPLC) is used to efficiently purify natural proteins or recombination fusion proteins with a hexahistidine tag using the principle of Ni-NTA IMAC

A summary of our findings We found that treatment of primary CD8 + T cells with MMA promoted an exhausted phenotype characterized by decreased proliferation, activation, and effector functions

Iron dysregulation is a hallmark of ferroptosis, where excess intracellular iron promotes the Fenton reaction, generating hydroxyl radicals and amplifying oxidative stress

Furthermore, we have not seen evidence that the equipment used during drug preparation results in discarded amounts exceeding 10 percent

Falc et al., 2006)